The applications that do not work on Catalina are AutoConfig; EndNote X9; GeneQuest; GenVision Utility; SeqMan Pro; LastPass (Basically all the 32-bits applications).

You can check following the link below. https://www.businessinsider.com/apple-macos-catalina-how-to-check-apps-compatible-32bit-64bit-2019-10#if-you-want-to-make-sure-an-app-will-be-compatible-with-macos-catalina-apples-advice-is-to-contact-the-apps-developer-7

Some useful features on Catalina:

1. Revert after a bad upgrade. If you install a software update and realized that it will break other software, you can restore Mac back to the prior state by using macOS Recovery (enter macOS Recovery following the Reimage Doc).